The transient receptor potential vanilloid 2 (TRPV2) ion channel is activated by a chemical ligand (2-aminoethoxydiphenyl borate; 2-APB), noxious heat and mechanical stimulation.In a heterologous mammalian swan river daisy seeds cell expression system, the oxidant chloramine T (ChT) sensitizes TRPV2 activation in response to 2-APB and heat by oxidation of methionine residues at positions 528 and 607 in rat TRPV2.Here, we used a Xenopus oocyte expression system to determine whether ChT-mediated oxidation can also sensitize remedy essentials zinc paste TRPV2 to mechanical stimulation.In this system, we confirmed that ChT sensitized TRPV2 activation in response to 2-APB and heat, but we detected no sensitization to mechanical stimulation.
This result suggests that the activation mechanism of TRPV2 by a chemical ligand and heat differs from that for mechanical stimulation.Further, we demonstrated that two-electrode voltage clamp recording in the Xenopus oocyte expression system is an excellent format for high throughput analysis of oxidization of redox-sensitive TRP channels.